Abstract
BackgroundThe plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete.ResultsA new temperature-sensitive white stripe leaf 9(wsl9) rice mutant is described. The mutant develops white stripes during early leaf development, but becomes green after the three-leaf stage under field conditions. The wsl9 mutant was albinic when grown at low temperature. Gene mapping of the WSL9 locus, together with complementation tests indicated that WSL9 encodes a novel protein with an HNH domain. WSL9 was expressed in various tissues. Under low temperature, the wsl9 mutation caused defects in splicing of rpl2, but increased the editing efficiency of rpoB. Expression levels of plastid genome-encoded genes, which are transcribed by plastid-coded RNA polymerase (PEP), chloroplast development genes and photosynthesis-related genes were altered in the wsl9 mutant.ConclusionWSL9 encodes an HNH endonuclease domain-containing protein that is essential for early chloroplast development. Our study provides opportunities for further research on regulatory mechanisms of chloroplast development in rice.
Highlights
The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete
The wsl9 mutant was albinic when grown at low temperature
Phenotypic Characteristics of the wsl9 Mutant The mechanisms of chloroplast development were studied in a white-striped leaf mutant wsl9, identified following ethyl methane sulfonate (EMS) mutagenesis of japonica cultivar Ninggeng 3
Summary
The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete. Photosynthesis is a complex process that determines yield. Chloroplasts have crucial roles in plant development and growth by utilization of CO2 and biosynthesis of carbon skeletons as well as other physiological processes (Sakamoto et al 2008; Jarvis and López-Juez 2013). It is Nuclear-coded RNA polymerase (NEP) and plastidcoded RNA polymerases (PEP) together determine the biosynthesis and function of chloroplasts (Tiller and Bock 2014). NEP is a eukaryotic single subunit RNA polymerase encoded by nuclear genes but is located in the plastids (Liere et al 2011). RNA splicing is a processing event in which the introns of a precursor messenger RNA
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