WS17‐03Relationship between ultrasound‐determined follicular size and DNA ploidy of human granulosa cells

Abstract

BackgroundThe possibility that the hyperstimulatory effect of gonadotropins may be important etiologically in the genesis of some cases of ovarian cancer is still under discussion. Few flow and image cytometric studies report on DNA aneuploid granulosa cells in follicular fluid from women undergoing in vitro fertilization (IVF). The reports are very controversial. The aim of the study was to analyse relationship between ultrasound‐determined follicular size and DNA ploidy of granulosa cells from gonadotropin‐stimulated follicles.MethodThe granulosa‐luteal cells were obtained from women undergoing an IVF‐ET program at the University Department of obstetrics and Gynecology Ljubljana. Seventy aspirates of individual follicles (transvaginal determined size, range 15 mm – 30 mm in diameter) from gonadotropin‐stimulated cycles were analysed. Cytospins were prepared and fixed in delaunay fixative. All the slides were stained by the feulgen thionin method. Image DNA analyses was performed on automated high‐resolution image cytometer. The DNA index was calculated to define DNA ploidy.ResultsAll granulosa cell samples were found to be diploid.ConclusionRegardless of different ultrasound‐determined size of follicles, all analysed granulosa cells have diploid DNA content.AcknowledgementsSupported by grants from ministry of Research and technology republic of Slovenia (No. j3‐0428–032–98).