WS13.1 Microevolution of Pseudomonas aeruginosa in cystic fibrosis lungs

Abstract

Objectives Serial P. aeruginosa isolates in half year intervals from 35 CF patients at our local CF clinic who became colonized with P. aeruginosa in the 1980s were selected. The microevolution of P. aeruginosa in CF lungs was investigated in the six patients with the mildest and the six patients with the most severe course of their chronic P. aeruginosa infection. Methods Serial isolates were genotyped with a customized microarray. Sequential isolates of the initially colonizing clone were then subjected to whole genome sequencing by SOLiD5500 technology. Nucleotide variations compared to the PA14 genome were extracted, filtered, annotated and used for the reconstruction of clades. The 250 sequenced bacterial isolates were characterized in mutation rates, morphology, motility and secretion of virulence effectors. Results Exopolysaccharide biosynthesis, antimicrobial resistance and global regulators of lifestyle and metabolism are the most common functional categories whose genes were hit by mutations in the CF lungs. Microevolution was not uniform. The P. aeruginosa clone inhabiting severely affected lungs repetitively generated descendants with stop mutations or drastic amino acid changes in key genes of lifestyle, but these loss-of-function mutants were not recovered later. In contrast, P. aeruginosa clones predominantly acquired benign amino acid substitutions in patients who maintained a normal function of their chronically colonized lungs for up to 30 years. Conclusion Modes of microevolution of P. aeruginosa in CF lungs are associated with the severity of the chronic lung infection. Supported by DFG (SFB900) and Christiane Herzog Stiftung.