Wound-inducible ANAC071 and ANAC096 transcription factors promote cambial cell formation in incised Arabidopsis flowering stems

Keita Matsuoka,Kyomi Shibata,Yuki Matsukura,Masashi Asahina,Shinobu Satoh,Naoyuki Nozawa,Yoshiki Kawajiri,Hiromi Iino,Ryosuke Sato,Yuki Kondo

doi:10.1038/s42003-021-01895-8

Keita Matsuoka, Kyomi Shibata + Show 8 more

Open Access

https://doi.org/10.1038/s42003-021-01895-8

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Abstract

ANAC071 and its homolog ANAC096 are plant-specific transcription factors required for the initiation of cell division during wound healing in incised Arabidopsis flowering stems and Arabidopsis hypocotyl grafts; however, the mechanism remains mostly unknown. In this study, we showed that wound-induced cambium formation involved cell proliferation and the promoter activity of TDR/PXY (cambium-related gene) in the incised stem. Prior to the wound-induced cambium formation, both ANAC071 and ANAC096 were expressed at these sites. anac-multiple mutants significantly decreased wound-induced cambium formation in the incised stems and suppressed the conversion from mesophyll cells to cambial cells in an ectopic vascular cell induction culture system (VISUAL). Our results suggest that ANAC071 and ANAC096 are redundantly involved in the process of “cambialization”, the conversion from differentiated cells to cambial cells, and these cambium-like cells proliferate and provide cells in wound tissue during the tissue-reunion process.

Highlights

ANAC071 and its homolog ANAC096 are plant-specific transcription factors required for the initiation of cell division during wound healing in incised Arabidopsis flowering stems and Arabidopsis hypocotyl grafts; the mechanism remains mostly unknown
To reveal the function of ANAC071 and ANAC096 for the regeneration of wounded tissue, we investigated tissue localization and morphological change in the incised flowering stem, and we concluded that ANAC071 and ANAC096 are essential for wound-induced cambium formation from dedifferentiated cells before the initiation of cell division during the tissue-reunion process
Several previous studies reported that the parenchyma cells around the wound site divided and redifferentiated into xylem/phloem vessels to connect the vascular system after incision in eudicots[27,28,29]

Summary

Introduction

ANAC071 and its homolog ANAC096 are plant-specific transcription factors required for the initiation of cell division during wound healing in incised Arabidopsis flowering stems and Arabidopsis hypocotyl grafts; the mechanism remains mostly unknown. We showed that wound-induced cambium formation involved cell proliferation and the promoter activity of TDR/PXY (cambium-related gene) in the incised stem. Auxin promotes cambium formation and differentiation into xylem and phloem elements around the wound site in tobacco stem and cactus graft[1,2]. Auxin appears to trigger wound-induced cambium formation and the redifferentiation of xylem/phloem vessel elements during the process of wound repair. The fundamental molecular mechanism of wound-induced cambium formation and redifferentiation during wound healing would be nearly the same as that of cambial proliferation and differentiation during the process of growth and development because genes expressed in the cambium, xylem, and phloem were highly induced during the process of hypocotyl grafting[10]. WITH XYLEM (PXY)), which localizes in the cellular membrane of cambial cells, and promotes its own cell proliferation[12,13]

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