Abstract
We analyzed the potential antibacterial effects of two different PdB against methicillin-resistant S. aureus and P. aeruginosa. The third-degree burn wound healing effects of PdB was also studied. Blood samples were obtained from 10 healthy volunteers and biological assays of the PdB were performed and the antimicrobial activity against MRSA and P. aeruginosa was determined using disk diffusion (DD), broth microdilution (BMD), and time-kill assay methods. 48 Wistar albino rats were burned and infected with MRSA. Two groups were injected PdB, the control groups were treated with plasma and received no treatment respectively. In the next step, the rats were euthanized and skin biopsies were collected and histopathologic changes were examined. The results of DD and BMD showed that both PdB performed very well on MRSA, whereas P. aeruginosa was only inhibited by F-PdB and was less susceptible than MRSA to PdBs. The time-kill assay also showed that F-PdB has an antibacterial effect at 4 hours for two strains. Histopathological studies showed that the treated groups had less inflammatory cells and necrotic tissues. Our data suggest that PdB may possess a clinical utility as a novel topical antimicrobial and wound healing agent for infected burn wounds.
Highlights
We analyzed the potential antibacterial effects of two different Platelet-derived biomaterial (PdB) against methicillin-resistant S. aureus and P. aeruginosa
The results of our disk diffusion (DD) and minimum inhibitory concentration (MIC) methods showed that the PdB performed very well on Methicillin-resistant S. aureus (MRSA) but the results were different for P. aeruginosa and only freeze- PdB” (F-PdB) had an inhibitory effect on this bacterium
It seems that the more resistance of P. aeruginosa compared to S. aureus is further depended on differences in cell membrane charge and composition that reduces the interaction of platelet antimicrobial agents with the cell surface of gram-negative bacteria[25]
Summary
This indicated that the PdBs were not contaminated The results of this method showed that both CaCl2-PdB and F-PdB were able to reduce the number of MRSA bacteria compared to the control group. CaCl2-PdB and F-PdB antimicrobial effects did not differ significantly from each other (p > 0.05) Both of the PdBs decreased the number of bacteria on days 3 and 7 compared to the control group (which did not receive any treatment). The qualitative assessment showed that in the wounds treated by F-PdB and CaCl2-PdB the number of inflammatory cells was reduced This phenomenon causes new angiogenesis, higher collagen deposition and reepithelization earlier in the treated groups than the control group (Fig. 4). There was no difference in collagen production, reduction of inflammatory cells and angiogenesis between the F-PdB and CaCl2-PdB treated groups
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