Wound Healing-Promoting and Melanogenesis-Inhibiting Activities of Angelica polymorpha Maxim. Flower Absolute In Vitro and Its Chemical Composition.

Su-Yeon Lee,Mi-Jung Kim,Kyung-Jong Won,Nan-Young Kim,Hwan-Myung Lee,Yu-Rim Won,Do-Yoon Kim

doi:10.3390/molecules26206172

Su-Yeon Lee, Mi-Jung Kim + Show 5 more

Open Access

https://doi.org/10.3390/molecules26206172

Copy DOI

Abstract

Angelica polymorpha Maxim. (APM) is used in traditional medicine to treat chronic gastritis, rheumatic pain, and duodenal bulbar ulcers. However, it is not known whether APM has epidermis-associated biological activities. Here, we investigated the effects of APM flower absolute (APMFAb) on responses associated with skin wound healing and whitening using epidermal cells. APMFAb was obtained by solvent extraction and its composition was analyzed by GC/MS. Water-soluble tetrazolium salt, 5-bromo-2′-deoxyuridine incorporation, Boyden chamber, sprouting, and enzyme-linked immunosorbent assays and immunoblotting were used to examine the effects of APMFAb on HaCaT keratinocytes and B16BL6 melanoma cells. APMFAb contained five compounds and induced keratinocyte migration, proliferation, and type IV collagen synthesis. APMFAb also induced the phosphorylations of ERK1/2, JNK, p38 mitogen-activated protein kinase, and AKT in keratinocytes. In addition, APMFAb decreased serum-induced B16BL6 cell proliferation and inhibited tyrosinase expression, melanin contents, and microphthalmia-associated transcription factor expression in α-melanocyte-stimulating hormone-stimulated B16BL6 cells. These findings demonstrate that APMFAb has beneficial effects on skin wound healing by promoting the proliferation, migration, and collagen synthesis of keratinocytes and on skin whitening by inhibiting melanin synthesis in melanoma cells. Therefore, we suggest that APMFAb has potential use as a wound healing and skin whitening agent.

Highlights

Introduction iationsSkin wound healing is a complex process and comprised of inflammation, proliferation, and remodeling phases [1]
We investigated the effects of Angelica polymorpha Maxim. (APM) flower absolute (APMFAb) on skin wound healing- and whitening-related events in vitro using human immortalized keratinocytes (HaCaT cells) and melanoma cells (B16BL6 cells), respectively, and the mechanisms involved
To determine whether APMFAb affects skin wound healing, we investigated its effects on keratinocyte migration and proliferation, which are known to play important roles in the healing process [12]

Summary

Introduction

Skin wound healing is a complex process and comprised of inflammation, proliferation, and remodeling phases [1]. The skin healing process involves interactions between multiple factors such as different cell types in skin, growth factors, cytokines, and extracellular matrix (ECM) [1,2]. The occurrence of a skin wound causes keratinocytes, which make up most of the epidermis, to proliferate and migrate to the damaged area to facilitate re-epithelialization [1]. The migratory and proliferative activities of keratinocytes are activated by epidermal growth factor (EGF) and other growth factors. Collagen is involved in all phases of wound healing and plays important roles during the proliferative and remodeling phases of wound healing [1]

Methods

Results

Conclusion