Abstract

A wound is a complicated bioprocess resulting in significant tissue damage, which is worsened by a secondary bacterial infection, commonly Pseudomonas aeruginosa and Staphylococcus aureus. The goal of our study was to investigate the metabolic profile and possible wound-healing effect of Sanguisorba officinalis roots rhoifolin rich fraction (RRF). The LC-ESI-MS/MS analysis of S. officinalis roots crude ethanol extract resulted in a tentative identification of 56 bioactive metabolites, while a major flavonoid fraction was isolated by column chromatography and identified by thin-layer chromatography coupled with electrospray ionization/mass spectrometry (TLC-ESI/MS), where rhoifolin was the major component representing 94.5% of its content. The antibiofilm activity of RRF on the mono-species and dual-species biofilm of P. aeruginosa and S. aureus was investigated. RRF exhibited inhibitory activity on P. aeruginosa and S. aureus mono-species biofilm at 2× minimum inhibitory concentration (MIC) and 4× MIC values. It also significantly inhibited the dual-species biofilm at 4× MIC values. Moreover, the wound-healing characteristics of RRF gel formulation were investigated. Rats were randomly allocated into four groups (eight rats in each): Untreated control; Blank gel; Betadine cream, and RRF gel groups. Animals were anesthetized, and full-thickness excisional skin wounds were created on the shaved area in the dorsal skin. The gels were topically applied to the wound’s surface daily for 10 days. The results demonstrated that RRF had a promising wound-healing effect by up-regulating the platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), keratinocyte growth factor (KGF), and fibronectin, while metalloproteinase-1 (MMP-1), interleukin-6 (IL-6), IL-1β, and nitric oxide (NO) levels were suppressed. It also enhanced the immune staining of transforming growth factor (TGF-β) and improved histopathological findings. Furthermore, it displayed an immunomodulatory action on lipopolysaccharide-induced peripheral blood mononuclear cells. Hence, the wound-healing effect of rhoifolin was confirmed by supporting re-epithelization, angiogenesis, antibacterial, immunomodulatory, and anti-inflammatory activities.

Highlights

  • Wounds represent a major health problem in which the skin is damaged by numerous overlapping processes such as hemostasis, inflammation, proliferation, and remodeling [1,2].Wound healing is a multi-step process that requires the cooperation of various cell types such as keratinocytes, fibroblasts, endothelial cells, macrophages, and platelets [3]

  • The results of metabolic profiling revealed that luteolin-6-C-glucoside (P17), isookanin7-glucoside (P20), and rhoifolin (P26) were among the major peaks detected in negative mode, while 1-O-β-D -glucopyranosyl sinapate (P5), catechin (P15), and 3,30,40,5-tetrahydroxy7-methoxyflavone (P32) were among those in the positive counterpart (Table 1)

  • rhoifolin rich fraction (RRF) isolated from S. officinalis L. radix was explored for its wound-healing properties for the first time to the best of our knowledge

Read more

Summary

Introduction

Wounds represent a major health problem in which the skin is damaged by numerous overlapping processes such as hemostasis, inflammation, proliferation, and remodeling [1,2]. Wound healing is a multi-step process that requires the cooperation of various cell types such as keratinocytes, fibroblasts, endothelial cells, macrophages, and platelets [3]. Bacteria and fungus can contaminate wounds, slowing the healing process. Topical antimicrobials are prescribed in an attempt to limit the possible infection of deeper body tissues and blood circulation, which could lead to sepsis [4]. Some topical antibiotic preparations may have cytotoxic effects in addition to antibiotic resistance, hindering and complicating wound healing. The use of medicinal plant preparations can exert beneficial effects due to their biocompatibility, wound healing, and antimicrobial properties

Objectives
Methods
Results
Discussion
Conclusion
Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.