Wound healing potential of formulated ointment by using virgin coconut oil in combination with Ethanol extract of Artocarpus lakoocha Roxb. leaves

Abstract

Wound healing is a complex process involving many cells consisting of four phases namely hemostasis, inflammation, proliferation, and remodeling. The usage of natural resources that have pharmacological features can hasten the wound's physiological healing procedure. The example of natural resources is virgin coconut oil (VCO) and ethanol extract of Artocarpus lakoocha Roxb. leaves (EEAL) which have the potential for wound healing. This study aimed to examine the combination of VCO and EEAL in an ointment formulation for wound healing in male Wistar rats. The ointment was divided into five formulas with different concentrations of VCO and EEAL and underwent stability and homogeneity evaluation. In vivo animal testing was divided into five groups according to the number of formulas. The wound healing activity was evaluated by using white male rats in the aspect of wound healing percentage and epithelial diameter from a histopathology study. The results showed that all formulas are stable and F3 by using the ratio of 3:1 (VCO: EEAL) has the best wound healing activity in the aspect of wound healing percentage and epithelial diameter compared to the other groups. In conclusion, our findings suggest that the VCO and EEAL in ointment preparation have great potential for wound healing activity.