Wound healing phytoconstituents from seed kernel of Entada pursaetha DC. and their molecular docking studies with glycogen synthase kinase 3-β

Abstract

The present study was aimed at isolation of phytoconstituents from the seed kernel of Entada pursaetha DC. and screened for their wound healing activity by excision, incision, and dead space wound models on Swiss Albino Wistar rats. In correlation to in vitro wound healing activity, all phytoconstituents were subjected to molecular docking studies for the inhibition of glycogen synthase kinase 3-β (GSK3-β) protein, a main target for wound healing activity by Wnt signaling pathway. Among the phytoconstituents, entagenic acid showed good wound healing activity. In excision wound model, entagenic acid showed significant activity compared with other two phytoconstituents (Mean time of epithelialization in days is 18.08 ± 0.01); in incision wound model, it showed tremendous activity with skin breaking strength of 655.67 ± 4.14 than nitrofurazone (683.33 ± 33.33) and also in dead space wound model entagenic acid showed very significant activity. Also in molecular docking studies, entagenic acid showed minimum binding (−8.79 kJ mol−1) and docking (−8.95 kJ mol−1) energy with good affinity toward active pocket, thus entagenic acid may be considered as good inhibitor of GSK3-β.