Abstract
Background and Aim:Momordica charantia is mainly characterized by its antimicrobial and antioxidant properties. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice.Materials and Methods:Acetonic extract of M. charantia was prepared and incorporated into gel and cream formulations. Mus musculus Balb/c (n=30) with induced injury were distributed into five groups: Group I (control – day 7), Group II (control – day 14), Group III (1% gel – day 7), and Group IV (1% gel – day 14) to which 1% M. charantia gel was dermally applied daily for 7 and 14 days, respectively, Group V (1% cream – day 7) and Group VI (1% cream – day 14) to which of M. charantia 1% cream were dermally applied daily for 7 and 14 days, respectively. Time of wound closure was determined during the experimentation; rats were euthanized with sodium pentobarbital 60 mg/kg/pc v.ip. for obtaining skin samples for histopathological analysis.Results:Groups IV and VI showed a higher percentage of wound closure on day 14, and in histopathological analysis, effect was greater in Group VI with the presence of fibroblasts and abundant collagen and elastic fibers.Conclusion:M. charantia gel and cream showed wound healing activity on induced wounded mice; the most effective treatment was M. charantia 1% cream formulation.
Highlights
Wound healing is a complex and dynamic process that involves both local and systemic cellular and biochemical responses, which is supported by many cellular events that must be closely coordinated to efficiently repair damaged tissue [1,2]
Thirty M. musculus Balb/c were randomly distributed into six experimental groups with five specimens per group: Group I and Group II that did not receive any treatment, Group III (1% gel – day 7) and Group IV (1% gel – day 14) to which 1% gel of M. charantia was applied daily by topical route for 7 and 14 days, respectively; Group V (1% cream – day 7) and Group VI (1% cream – day 14) to which 1% cream of M. charantia was applied daily for 7 and 14 days, respectively
Histopathological changes in mice skin in Groups I and II showed little differentiation from basal cell layer and in connective tissue, a scarce fibroblast migratory activity was shown as signs of progressive physiological healing (Figures-2a and b)
Summary
Wound healing is a complex and dynamic process that involves both local and systemic cellular and biochemical responses, which is supported by many cellular events that must be closely coordinated to efficiently repair damaged tissue [1,2]. This process begins with a fibroblastic stage where the wound area contracts [3]. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice
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