Abstract
This study aims to carry out the extraction and isolation of bioactive compounds from the peel of Punica Granatum Linn and investigate the wound healing activity of isolated compounds. Soxhlet extraction of P. Granatum peel powder was done with ethanol as solvent. The isolation of phytochemical constituents, Punicalin and Punicalagin present in the obtained extract, was done with the help of column chromatography using n-butanol and ethyl acetate fractions. The column was packed with silica gel, and 100-200 mesh was used as a stationary phase separately at different stages of the isolation process. All fractions obtained from column chromatography were subjected to HPLC, LCMS, FTIR, 1HNMR studies to identify and confirm the obtained isolated bioactive. The study was further extended to assess the wound healing activity of isolated punicalin and punicalagin by using in vitro excision and incision models. The activity of prepared alcoholic extract and isolated bioactive was compared to standard 10 % w/w povidone-iodine ointment. The highest wound healing strength observed was 201.83 ± 4.98 for isolated 10% w/w punicalagin ointment in the incision wound model. Also, in the excision wound model, the highest % reduction in the wound was observed on the 15th day as 88 ± 0.78, which was optimum compared to the standard with a % reduction in the wound as 92 ± 0.91. As a result, bioactive punicalagin and punicalin obtained from methanol extract of Punica Granatum peel powder can be used as potent phytoconstituents and wound healing agents for pharmaceutical preparations
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