Abstract
The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This methodwas used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched wounded area. The scratched area was monitored after 24 hours of wound closure in the Biofield Energy Treated HFF-1 cells and the Biofield Energy Treated DMEM groups, and the representative photomicrographs were taken in each wells using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEMwassignificantly higher percentage of fibroblast migration i.e. 51.8%, while the migration was altered in the Biofield Energy Treated HFF-1 cell line compared to the baseline control group. In addition to, the percentage of scratch area was significantly decreased by 2.7% in the Biofield Energy Treated DMEM group, while it was increased by 12% in the Biofield Treated cells group compared to the baseline control group. Overall, the experimental results concluded that The Trivedi Effect® has the significant capacity and wide implications in wound healing activityvia cell culture media, DMEM as compared with the HFF-1 cell line directly. Biofield Energy Healing would be a complementary and alternative medicine that can be used against burn injury cases, acute wound, skin regeneration, eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.
Highlights
“Wound” results from an opening or breaking of the skin results in a physical injuries and “wound healing”, which a complex multifactorial process is including integrated cell responses to injury
Biofield Energy culture medium were received Biofield Energy Treatment (Con-Therapies have been reported with therapeutic potentials in case sciousness Energy Healing Treatment-The Trivedi Effect®) by of an improved personal well-being in cancer patient[8], better a renowned Biofield Energy Healer, Mahendra Kumar Trivedi, functional ability in arthritis patient[9], reduced pain and anxi- who participated in this study under laboratory conditions for ~3 ety[10], and in wound healing[11,12]
The present study evaluated the effect of Biofield Energy Treatment (The Trivedi Effect®) on (Human foreskin fibroblast-1) HFF-1 cell line and Dulbecco’s Modified Eagle Medium (DMEM) using scratch assay for quantitative determination of
Summary
“Wound” results from an opening or breaking of the skin results in a physical injuries and “wound healing”, which a complex multifactorial process is including integrated cell responses to injury. The cells were sub-cultured by trypsinization followed by favor of Biofield Energy Healing against cancer research that splitting the cell suspension into fresh flasks and supplementing suggest better results of Energy Medicine as compared with oth- with fresh cell growth medium. The present study evaluated the effect of Biofield Energy Treatment (The Trivedi Effect®) on (Human foreskin fibroblast-1) HFF-1 cell line and Dulbecco’s Modified Eagle Medium (DMEM) using scratch assay for quantitative determination of. Energy Treatment, the medium and the cell line were used for estimation of in vitro wound healing potential using scratch assay. Group III was referred as HFF-1 cells in the Biofield Energy (known as The Trivedi Effect®) Treated DMEM. The. Chemicals and Reagents: Dulbecco’s Modified Eagle Medium scratched area was monitored after 24 hours for closure (DMEM), Fetal Bovine Serum (FBS), and Recombinant Human of wound area.
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