Abstract
Objectives To analyze the effect of topical application of a photopolymerizable hydrogel based on methacrylated gelatin containing 2% ethanolic extract of Himatanthus bracteatus (GelMA-HB) on wound healing in rodents. Study Design Standard-sized (0.8 cm diameter) round-shaped wounds were made in the back of Wistar rats treated with photopolymerizable hydrogel based on methacrylated gelatin (GelMA), GelMA-HB, and saline (CTR). The animals were euthanized after 3, 7, and 14 days. Wound retraction index (WRi), wound healing histologic grading (WHHG), and immunohistochemical expression of α-SMA and CD105 were assessed for myofibroblastic differentiation (MFd) and microvascular density (MVd) analysis, respectively. Results The WRi and WHHG in GelMA-HB were significantly greater than in GelMA (P < .05) and CTR (P < .01) over the full time course of the experiment. Both the MFd and MVd were greater in GelMA-HB than in CTR on day 3 (P < .05) and day 7 (P < .001). Conclusions The GelMA-based formulation containing ethanolic extract of H. bracteatus improved wound healing in a rodent model, and such biological effects might be related to the modulation of myofibroblastic and microvascular differentiation over the course of wound healing. To analyze the effect of topical application of a photopolymerizable hydrogel based on methacrylated gelatin containing 2% ethanolic extract of Himatanthus bracteatus (GelMA-HB) on wound healing in rodents. Standard-sized (0.8 cm diameter) round-shaped wounds were made in the back of Wistar rats treated with photopolymerizable hydrogel based on methacrylated gelatin (GelMA), GelMA-HB, and saline (CTR). The animals were euthanized after 3, 7, and 14 days. Wound retraction index (WRi), wound healing histologic grading (WHHG), and immunohistochemical expression of α-SMA and CD105 were assessed for myofibroblastic differentiation (MFd) and microvascular density (MVd) analysis, respectively. The WRi and WHHG in GelMA-HB were significantly greater than in GelMA (P < .05) and CTR (P < .01) over the full time course of the experiment. Both the MFd and MVd were greater in GelMA-HB than in CTR on day 3 (P < .05) and day 7 (P < .001). The GelMA-based formulation containing ethanolic extract of H. bracteatus improved wound healing in a rodent model, and such biological effects might be related to the modulation of myofibroblastic and microvascular differentiation over the course of wound healing.
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