Wound, Adjacent Skin and Control Skin Radioactivty After Injection of 1,2-3H-Cortisol and 1,2-3H-Testosterone

Abstract

Either 3mg of 1,2- 3 H-cortisol (20 μc) or 5mg of 1,2- 3 H-testosterone (20 μc) was injected intramuscularly into young adult male rates, 1, 4 and 7 days after skin wounds were created by intradermal croton oil injection. Thirty minutes and 4 hours after injection of each labeled steroid, radioactivity was measured in wound tissue, in adjacent to the wound, in a contralateral control site and in plasma. The radioactivity obtained from the tissues is expressed in 3 ways: (1) as radioactivity extracted from fresh tissue; (2) as radioactivity calculated for dry fat-free tissue; (3) as radioactivity calculated for tissue water. The relative amount of radioactivity in wound, adjacent skin and control skin frequently varied with the manner of data expression. There was a difference between the distribution of radioactivity from cortisol and testosterone in control and adjacent skin. No distinct differences with wound age were observed in the distribution of either cortisol or testosterone between croton oil lesions, adjacent to the wound and control skin. Tissue water radioactivities indicated that none of 3 tissues investigated concentrated cortisol and/or its metabolites. However, tissue water radioactivities of control and adjacent skin of rats given testosterone were similar to or greater than plasma tissue water radioactivities.