Abstract
In the course of the study we have worked out the methodology of differential spectrophotometric determination of flavonoids in raw Leontodon autumnalis L. after the formation of a colored complex with a solution of aluminum chloride. We have established optimal conditions for complexation and extraction of flavonoids from plant raw materials under study. We have established that the most complete extraction of flavonoids is achieved with fineness of 1 mm by extraction with ethyl alcohol of 70% for 45 minutes. The maximum value of the optical density is achieved 30 minutes after addition of 2 ml of a solution of aluminum chloride 2%.
Highlights
WORKING OUT AND VALIDATION OF THE METHOD OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE HERB OF LEONTODON AUTUMNALIS L
We have suggested a method of spectrophotometry determination for the quantitative determination of flavonoids sum in raw materials of Leontodon autumnalis L. after preliminary conduction of reaction of complexation with solution of aluminum chloride in SF2000 spectrophotometer
Following the worked out method we have established a content of flavonoids in the herb of Leontodon autumnalis L., stored up in different habitats
Summary
Correlation coefficient amounted to 0.999954, the worked out method can be used for the quantitative determination of flavonoid sum in terms of cynaroside within a given range of concentrations. Acceptance criterion was expressed by the value of relative standard deviation, which should not exceed 10% (table 4)
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