WOOD-DESTROYING PROPERTIES OF FOMITOPSIS PINICOLA (SW.) P. KARST. FROM MIDDLE SIBERIA

Abstract

Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'–56°22' N; 92°10'–92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1–3,3 mm / day; the growth coefficient is 10–42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05–0,39 U / ml with a maximum of 7–9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood – the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lignin – in 1,2 times.