Wnt5a might regulate the invasion and proliferation of papillary thyroid carcinoma cells through receptor tyrosine kinase-like orphan receptor 2

Abstract

Objective To detect the effect of receptor tyrosine kinase-like orphan receptor 2 (ROR2) low-expression combining Wnt5a over-expression on the proliferation and invasion abilities of papillary thyroid carcinoma cells. Methods The proliferation ability of papillary thyroid carcinoma cells with ROR2 low-expressed combining Wnt5a over-expressed was detected by methyl thiazol tetrazolium (MTT). The clone formation ability of papillary thyroid carcinoma cells with ROR2 low-expressed combining Wnt5a over-expressed was detected by colony formation assay. The invasion ability of papillary thyroid carcinoma cells with ROR2 low-expressed combining Wnt5a over-expressed was detected by Transwell. Results MTT assay results showed that the A values detected on the fourth day of the K1 and BCPAP cells of the control groups (0.452±0.005, 0.432±0.003) were significantly lower than those of the Wnt5a groups (0.563±0.004, 0.567±0.006) but higher than those of the Si-ROR2 groups (0.404±0.003, 0.389±0.005) (t=-30.026, -34.857, 14.258, 12.773; P=0.000). The A values detected on the fourth day of the K1 and BCPAP cells of the co-stained groups (0.501±0.005, 0.496±0.003) were distinctively lower than those of the Wnt5a groups (t=-16.771, -19.545; P=0.000). The colony formation assay results showed that the numbers of K1 and BCPAP cell clones of the control groups (101.00±5.43, 112.00±4.11) were distinctively lower than those of the Wnt5a groups (156.00±4.34, 168.00±4.55) but higher than those of the si-ROR2 groups (62.00±4.45, 68.00±3.99) (t=-13.704, -15.819, 9.622, 13.304; P=0.000). The clone numbers of K1 and BCPAP cells of the co-stained groups (121.00±4.44, 128.00±3.89) were significantly lower than those of the Wnt5a groups (t=-9.764, -11.574; P=0.001, 0.000). The Transwell assay demonstrated that the numbers of K1 and BCPAP cells passing through the basement membrane of the control groups [(52.90±2.36), (68.40±1.01) cells] were significantly lower than those of Wnt5a groups [(164.30±4.35), (87.70±1.28) cells] but higher than those of si-ROR2 groups [(31.30±2.17), (39.60±1.34) cells, t=-38.829, -20.502, 11.533, 29.728; P=0.000]. The numbers of K1 and BCPAP cells passing through the basement membrane of the co-stained group [(83.50±1.42), (65.90±2.11) cells] were distinctively lower than those of the Wnt5a groups (t=-31.986, -15.300; P=0.000). Conclusion Wnt5a may regulate the proliferation and invasion of thyroid papillary carcinoma cells through ROR2 signaling. Key words: Wnt5a; Receptor tyrosine kinase-like orphan receptor 2; Papillary thyroid carcinoma; Cell proliferation; Cell invasion