Abstract
WNK3 interacts with NCC.
Highlights
Dear Editor, NCC (Na-Cl cotransporter) is a cotransporter mainly distributed in the distal tubule of the kidney, functioning to reabsorb sodium and chloride ions from the tubular fluid into the cells of the renal distal convoluted tubule
We report the results of our co-immunoprecipitation experiments and confocal observations, confirming that WNK3 does interact with NCC and that the kinase domain of WNK3 plays an important role in the regulation of NCC
The results showed that WNK3 increased NCC expression both on the cell membrane and in the cytoplasm
Summary
Dear Editor, NCC (Na-Cl cotransporter) is a cotransporter mainly distributed in the distal tubule of the kidney, functioning to reabsorb sodium and chloride ions from the tubular fluid into the cells of the renal distal convoluted tubule. We report the results of our co-immunoprecipitation experiments and confocal observations, confirming that WNK3 does interact with NCC and that the kinase domain of WNK3 plays an important role in the regulation of NCC. To explore whether there is reaction between WNK3 and NCC protein, Cos-7 cells were transfected by GFP-NCC with or without HA-WNK3 and co-immunoprecipitation
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