Within-Population Genetic Structure in Beech (Fagus sylvatica L.) Stands Characterized by Different Disturbance Histories: Does Forest Management Simplify Population Substructure?

Andrea Piotti,Joukje Buiteveld,Myriam Heuertz,Stefano Leonardi,Sophie Gerber,Thomas Geburek,Pär K Ingvarsson,Koen Kramer,Cristina Vettori,Giovanni Giuseppe Vendramin

doi:10.1371/journal.pone.0073391

Abstract

The fine-scale assessment of both spatially and non-spatially distributed genetic variation is crucial to preserve forest genetic resources through appropriate forest management. Cryptic within-population genetic structure may be more common than previously thought in forest tree populations, which has strong implications for the potential of forests to adapt to environmental change. The present study was aimed at comparing within-population genetic structure in European beech (Fagus sylvatica L.) plots experiencing different disturbance levels. Five plot pairs made up by disturbed and undisturbed plots having the same biogeographic history were sampled throughout Europe. Overall, 1298 individuals were analyzed using four highly polymorphic nuclear microsatellite markers (SSRs). Bayesian clustering within plots identified 3 to 11 genetic clusters (within-plot θ ST ranged from 0.025 to 0.124). The proportion of within-population genetic variation due to genetic substructuring (F CluPlot = 0.067) was higher than the differentiation among the 10 plots (F PlotTot = 0.045). Focusing on the comparison between managed and unmanaged plots, disturbance mostly explains differences in the complexity of within-population genetic structure, determining a reduction of the number of genetic clusters present in a standardized area. Our results show that: i) genetic substructuring needs to be investigated when studying the within-population genetic structure in forest tree populations, and ii) indices describing subtle characteristics of the within-population genetic structure are good candidates for providing early signals of the consequences of forest management, and of disturbance events in general.

Highlights

Within-population genetic structure is shaped by the complex interplay of genetic and demographic factors
Our experimental setup allowed us to obtain a reliable set of parameters describing the within-population genetic structure of European beech in a wide variety of ecological and management conditions, spanning from undisturbed to highly managed stands
Two relevant results for the comprehension of within-population dynamics in F. sylvatica were achieved: i) despite the variety of conditions explored, we found that a large proportion of withinpopulation genetic variation is due to genetic substructuring in all plots, and ii) in plot pairs sharing the same bio-geographic history, disturbance explains most of the difference in the complexity of within-population genetic structure

Summary

Introduction

Within-population genetic structure is shaped by the complex interplay of genetic and demographic factors. It has recently been pointed out that the study of within-population genetic structure is a key prerequisite to correctly interpret the results of association genetic studies, since cryptic genetic structure can yield spurious statistical associations between genotypic and phenotypic traits [6,7]. The probability of such misinterpretations is high when studying forest tree populations because they have long been seen as large, random-mating units with minimal structure [8].

Objectives

Methods

Results

Conclusion