WITHDRAWN: Long non-coding RNA RP5-821D11.7 promotes proliferation, migration, and epithelial-mesenchymal transition in glioma and glioma stem-like cells

Muhammad Younis,Khawar Ali Shahzad,Sana Shaikh

doi:10.1016/j.pbiomolbio.2021.12.001

Muhammad Younis, Khawar Ali Shahzad + Show 1 more

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https://doi.org/10.1016/j.pbiomolbio.2021.12.001

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Abstract

Long noncoding RNA (lncRNA) has been recently revealed as a main regulatory molecule, which implicates many cellular functions. Studies showed that lncRNA abnormally expressed and involved in the progression and tumorigenesis of glioma. Present study identified a novel lncRNA associated with glioma, glioma stem-like cells (GSCs), and then revealed their potential functions. During the screening of lncRNAs, we investigated overexpression of lncRNA RP5-821D11.7 (lncRNA-RP5) in GSCs compared to glioma cells. Lentivirus-mediated shRNA for lncRNA-RP5 was constructed and transfected into glioma cells. Transfected stable glioma cells were transplanted into nude mice and tumor growth was observed. Knockdown of lncRNA-RP5 significantly inhibits proliferation, colony formation, migration and reduces epithelial-mesenchymal transition (EMT) by activating the Wnt/β-catenin pathway. Additionally, the results showed that lncRNA RP5 knockdown enhances cell apoptosis through endoplasmic reticulum stress. Therefore, this study may provide a better understanding about lncRNA-RP5 which revealed that it might be a potential therapeutic target in case of glioma progression and recurrence.

Highlights

Malignant glioma is the most frequent and aggressive neoplasm of the brain[1]
Knockdown assay through sh-RNA significantly suppressed the expression of Long noncoding RNA (lncRNA)-RP5 in (U87 and U251) and glioma stem-like cells (GSCs) (U87SCs and U251SCs) cell lines compared to NC (Fig. 1B, C)
The effect of lncRNA-RP5 knockdown on the proliferation of glioma and GSCs was detected by CCK-8 assay, which indicated that the proliferation rate was significantly inhibited in the sh-RP5 group compared to the negative control (Fig. 1D-G)

Summary

Introduction

Malignant glioma is the most frequent and aggressive neoplasm of the brain[1]. According to World Health Organization (WHO) grading criteria, gliomas can be divided into grade I-IV[2]. LncRNAs are RNA molecules with less than 200 nucleotides that function as RNA with little or no protein encoding[3, 4]. LncRNA has been implicated to play functional role in tumorigenesis and cancer growth[5, 6]. There is increasing evidence that lncRNA participates in a diversity of many biological processes. The mechanistic role and functional diversity of lncRNAs are currently a field of research investigation[7, 8]

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