Abstract
Ahead of Print article withdrawn by publisher.
Highlights
Alzira Regina Silva de Deus1, Geice Ribeiro Silva2, Luciano Silva Sena2, Fábio Barros Britto3, Débora Araújo de Carvalho1, Jorge Victor Gomes de Freitas4, and JoséLindenberg Rocha
The lowest degree of genetic variability was observed in the Farm 6, i.e. He (0.700), polymorphic information content (PIC)
Sheep farming is practiced throughout Brazil, with a higher concentration in the Northeast and South regions of the country focusing on meat production
Summary
A total of 257 DNA samples from animals raised in six farms were genotyped using a panel of 20 microsatellite loci. The R packages Ppoppr, ADEadegenet, Aape, Ppolysat, and Gggplot were used to construct a dendrogram and perform the principal coordinates analysis (PCoA). Peripheral blood samples were collected from 257 animals of the Santa Inês sheep breed raised in six flocks located in different municipalities of the states of Piauí(PI) and Maranhão (MA), in the Mid-North sub-region of Brazil, from October 2013 to April 2018 (Table 1). Prior to DNA extraction, the blood samples were stored at -20°C, in vacuum tubes containing 1% of EDTA anticoagulant. The genomic DNA extraction and purification were performed using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The reactions were tested and optimized in a volume of 16 μL, until the following profile was achieved: at least 2.5 mM dNTPs, 1 × of Tris-HCl/KCl buffer, 1.0 to–
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