WITHDRAWN: Design and evaluation of a novel Kaolin-chitosan/gold nanocomposite for the treatment of human lung cancer

Abstract

• Kaolin-CS/Au nanocomposite have antioxidant potentials against free radicals DPPH. • Kaolin-CS/Au nanocomposite have anti-human lung cancer properties. Kaolin-chitosan (Kaolin-CS) is an interesting bio composite with applications in formulating the anticancer supplements. Thus, the purpose of this study is to prepare Kaolin-CS/Au nanocomposite by adding gold nanoparticles (Au NPs) to modify Kaolin with chitosan. The significance of this study is the novel initiative to combine the gold nanoparticle with inexpensive kaolin clay and utilization of them as anticancer agent. Through transmission electron microscopy, we found that the presence of CS could effectively prevent the agglomeration of gold nanoparticles on the surface of Kaolin. The Kaolin-CS/Au nanocomposite were characterized by electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), elemental mapping, X-ray diffraction (XRD) and inductively coupled plasma-optical emission spectroscopy (ICP-OES). In the cellular and molecular part of the recent study, the treated cells with Kaolin-CS/Au nanocomposite were assessed by MTT assay for 48h about the cytotoxicity and anti-human lung carcinoma properties on normal (HUVEC) and lung carcinoma cell lines i.e. NCI-H661, NCI-H1563, and NCI-H1299. The IC50 of Kaolin-CS/Au nanocomposite were 138, 144, and 138 µg/mL against NCI-H661, NCI-H1563, and NCI-H1299 cell lines, respectively. The viability of malignant lung cell lines reduced dose-dependently in the presence of Au NPs-Kaolin nanocomposite.