WITHDRAWN: Cordycepin inhibits LPS-induced acute lung injury by inhibiting inflammatory response

Abstract

// Xian Jiang 1 , Zhuo Zhang 2 , Fang Wang 3 , Siwei Chen 4 , Xiaobin Wang 1 and Jicheng Wei 1 1 Department of anesthesiology, The Affiliated Hospital of College of pharmacy, Southwest medical university, Luzhou, Sichuan, China 2 Laboratory of pharmacology, College of pharmacy, Southwest medical university, Luzhou, Sichuan, China 3 Laboratory of Pharmaceutics, College of pharmacy, Southwest medical university, Luzhou, Sichuan, China 4 Laboratory of Science of Chinese Pharmacology, College of pharmacy, Southwest medical university, Luzhou, Sichuan, China Correspondence: Jicheng Wei, email: jichengwei8383_88@yeah.net Keywords : cordycepin; LPS; lung injury; NLRP3; NF-κB Received: August 23, 2017 Accepted: December 16, 2017 Epub: January 01, 2018 Abstract Inflammatory response has been reported to play an important role in the development of acute lung injury (ALI). Cordycepin is known to have several pharmaceutical effects, including anti-antioxidant, and anti-inflammation. But no reported on the effect of Cordycepin on ALI. The present study was aimed to measure the protective effect of cordycepin on lipopolysaccharide (LPS)-induced ALI in mice. The mice were treated with cordycepin 1 h before LPS stimulation. 7 h later, the bronchial alveolar lavage fluid (BALF) was collected to measure the levels of inflammatory cells and inflammatory cytokines. The lung tissues were collected to test the lung pathological changes, wet/dry radio, myeloperoxidase (MPO) activity and the expression of NF-κB and NLRP3 signaling pathways. The results showed that cordycepin significantly inhibited the TNF-α, IL-1β, IL-6 production, the total cells, neutrophils, and macrophages numbers in BALF, and suppressed the pathological changes, wet/ dry radio, and MPO activity in lung tissues that induced by LPS. For further research the protect mechanism of cordycepin on ALI, we found that cordycepin inhibited the activation of NF-κB and NLRP3 signaling pathways in LPS-induced ALI in mice. In conclusion, the results suggested that cordycepin inhibited LPS-induced inflammatory response through modulating NF-κB and NLRP3 signaling, and cordycepin may be an effective treatment to cure ALI.