WITHDRAWN: Analysis of the application value of molecular diagnostic technology of mycobacterium tuberculosis

Abstract

To evaluate the application value of molecular diagnostic technology for tuberculosis. Firstly, the sensitivity and specificity were compared between sputum smear and isothermal amplification (IA, Di’ao Biological Technology Co., Ltd, Guangzhou, China) with solid culture as the standard method. Secondly, the detection rate was compared between liquid culture and molecular diagnostic methods, including IA and Xpert MTB/RIF. Thirdly, the minimum detection limit of IA and Xpert MTB/RIF was compared. Finally, IA was used from 2019 to February 2020, and Xpert MTB/RIF was used from March 2020, the contribution of molecular diagnosis to the etiological diagnosis of tuberculosis in Lishui District in Nanjing was compared in 2019 and 2020. The sensitivity of IA and sputum smear was 100% and 54.55%, respectively ( P < 0.05), and the specificity was 97.66% and 98.44%, respectively( P >0.05). IA was more consistent with solid culture( Kappa =0.924) than smear( Kappa =0.624). The detection rates of IA, Xpert MTB/RIF and liquid culture were 75.00%, 77.50% and 77.50%, respectively ( P > 0.05). The minimum detection limit of Xpert MTB/RIF was lower than that of IA. In 2019 and 2020, molecular diagnosis accounted for 31.58% and 39.28% of positive etiology, respectively, and the incidence of positive molecular pathogens was 1.25/100,000 and 2.24/100,000, respectively. Molecular detection technology improves the detection rate of tuberculosis etiology. Each TB designated detection hospital can select molecular detection methods according to local conditions, which will help control the spread of tuberculosis.