Withdrawal: Active participation of cellular chaperone Hsp90 in regulating the function of rotavirus nonstructural protein 3 (NSP3).

Abstract

Active Participation of Cellular Chaperone Hsp90 in Regulating the Function of Rotavirus Nonstructural Protein 3 (NSP3)Journal of Biological ChemistryVol. 286Issue 22PreviewHeat shock protein 90 (Hsp90) has been reported to positively regulate rotavirus replication by modulating virus induced PI3K/Akt and NFκB activation. Here, we report the active association of Hsp90 in the folding and stabilization of rotavirus nonstructural protein 3 (NSP3). In pCD-NSP3-transfected cells, treatment with Hsp90 inhibitor (17-N,N-dimethylethylenediamine-geldanamycin (17DMAG)) resulted in the proteasomal degradation of NSP3. Sequence analysis and deletion mutations revealed that the region spanning amino acids 225–258 within the C-terminal eIF4G-binding domain of NSP3 is a putative Hsp90 binding region. Full-Text PDF Open Access VOLUME 286 (2011) PAGES 20065–20077 This article has been withdrawn by the authors. The Journal determined that there are similar repetitive features in the background area of Figs. 4B, 5B, 6B, and 7A and Fig. S4, but the authors do not agree to the raised concerns. The authors state that they provided uncropped scans of the autoradiograms from the corresponding and/or replicate experiments done at same time of the original experiments. The authors state that due to the tropical climate, the quality of the autoradiograms may have partially deteriorated as the work was done more than 10 years ago, but still they could not visualize any repetitive elements in the background. However, the authors could not provide the original, uncropped electronic copies of the scans that were used for preparation of the published images. The authors state that all available hard copy autoradiograms present in the laboratory records were reviewed and verified favorably by the institutional review committee and a third-party forensic laboratory. The authors state that they had provided the cropped tiff files from the final assembled figures used during manuscript submission as well as figures downloaded from the JBC website to the forensic laboratory. Copies of both of the reports were shared with the Journal. The authors affirm that all the experiments were performed appropriately and the concerns raised do not affect the accuracy of the results or the conclusions of the scientific work. However, in the interest of maintaining the accuracy in the published scientific literature, the authors wish to withdraw this article but plan to publish a new version of the article corroborating the findings of this work in the future.