Wine Components Normalize the Cytochrome P450 Content in the Liver and Kidneys of Rats Under Neurogenic Stress

Abstract

A majority of organic environmental contaminants need to undergo the metabolic activation to exert their biological effects (toxic, mutagenic, transforming, etc.). This activation is catalyzed by monooxygenase system, which functional element is cytochrome P450 superfamily. One of the important properties of monooxygenase system is the ability of induction under the influence of external stimuli. Xenobiotics, different physiological factors, and stress can act as these stimuli. In our work, we studied cytochrome P450 content in the organs of the experimental animals under acute neurogenic stress and under the influence of alcohol and grape polyphenols. The obtained results showed that stressed animals had the low liver cytochrome P450 content compared with intact animals. Ethanol administration caused increasing of cytochrome P450 content in the liver of intact and stressed rats. Polyphenolic concentrate Enoant normalized the cytochrome P450 content in liver, whereas grape wines Cabernet and Rkatsiteli significantly increased cytochrome P450 content in both intact and stressed animals. The prophylactic administration of polyphenol concentrates to control animals did not significantly influence the cytochrome P450 content in hepatocytes and in rat kidneys. The cytochrome P450 content significantly decreased in animal kidneys under emotional-pain stress, but this effect was attenuated by ethanol administration. Incubation of isolated hepatocytes in the presence of phenacetin was accompanied by increased levels of cytochrome P450 in the cells. Grape polyphenols reduced cytochrome P450 content in the cells in dose-dependent and environment (e.g. stress, ethanol administration)-dependent manners, which suggests their possible role in the cancer prevention.KeywordsAryl Hydrocarbon ReceptorEthanol AdministrationGrape WineGrape Seed ExtractStressed AnimalThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.