Abstract
Calcium imaging of neurons in monkeys making reaches is complicated by brain movements and limited by shallow imaging depth. In a pair of recent studies, Trautmann et al., 2021 and Bollimunta etal. (2021) present complementary solutions to these problems.
Highlights
Calcium imaging of neurons in monkeys making reaches is complicated by brain movements and limited by shallow imaging depth
Imaging was feasible only at the cortical surface (< 600 mm), the high spatial resolution opened a window onto the activity of deep-layer neurons via calcium signals in their apical dendrites
The basis of this impressive stability is not entirely clear, but one possibility is that the lens served as an anchor, tethering the brain to the overlying dura mater and skull. The strengths of both techniques are augmented by their compatibility with histological analysis. This analysis provides a detailed snapshot of the transcriptomic type, laminar position, and morphology of the recorded neurons and is a valuable complement to the dynamic activity captured by calcium imaging
Summary
Calcium imaging of neurons in monkeys making reaches is complicated by brain movements and limited by shallow imaging depth. Revealing relationships between neural activity and behavior requires longitudinal, stable recordings from multiple neurons simultaneously (or nearly so), ideally of known transcriptional type and location. Imaging was feasible only at the cortical surface (< 600 mm), the high spatial resolution opened a window onto the activity of deep-layer neurons via calcium signals in their apical dendrites.
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