Abstract
Today, leukemia is one of the biggest problems worldwide. The Wilms' tumor gene (WT1) and the vascular endothelial growth factor (VEGF) gene are highly expressed in patients with various cancers. This study concerned the relationship between expression of WT1 and VEGF in patients with acute leukemia. We evaluated expression of WT1 mRNA and VEGF mRNA using real-time quantitative RT-PCR in the peripheral blood (PB) of 8 newly diagnosed AML and 4 newly diagnosed ALL patients, serially monitored for 2 months. A further 12 normal PB samples served as controls. In the patient group, in comparison with the normal ranges, WT1 and VEGF gene expression was increased, the average values for the expression of these two genes being 0.2852±0.11 and 0.2029±0.018, respectively. While was no significant relevance between the two genes pre-treatment, a positive link between the two genes in 75% of patients with AML was noted during the procedure of chemotherapy, whereas in 75% of patients with ALL an antiparallel association was observed. Leukemia is associated with production of WT1, which may affect the expression of VEGF.
Highlights
The biggest human health problem in the world is cancer (Phipps et al, 2007)
Materials and Methods: We evaluated expression of Wilms’ tumor 1 (WT1) mRNA and vascular endothelial growth factor (VEGF) mRNA using real-time quantitative reverse transcription (RT)-PCR in the peripheral blood (PB) of 8 newly diagnosed AML and 4 newly diagnosed ALL patients, serially monitored for 2 months
Miyoshi et al concluded that tumors >2 cm demonstrated an orientation toward a rise (p=0.09) in WT1 (Miyoshi et al, 2002b)
Summary
The biggest human health problem in the world is cancer (Phipps et al, 2007). It is considered that cancer is the second cause of death, followed by deaths due to adverse cardiovascular events. According to the results of Cancer Department State of Non Communicable Disease Management Center in 2010 reported that the incidence of blood cancer rates among women and men in Iran was 1330 and 2131, respectively. These figures for women and men in the Khuzestan province during the same year were 148 and 203, respectively (Korosh et al, 2012). Results: In the patient group, in comparison with the normal ranges, WT1 and VEGF gene expression was increased, the average values for the expression of these two genes being 0.2852±0.11 and 0.2029±0.018, respectively. Conclusions: Leukemia is associated with production of WT1, which may affect the expression of VEGF
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Pacific journal of cancer prevention : APJCP
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
