Abstract
Background. Predominant use of the H1 and Gro1-4 genes of resistance to golden nematode (PGN) in potato breeding requires widening the gene pool of resistance to this pathogen.Materials and methods. Thirty-four genotypes of wild potatoes from North and South Americas, 14 interspecific hybrids, and 10 Russian potato cultivars were studied for PGN resistance. Screening for resistance to PGN pathotype Ro1 and molecular screening for the presence of H1 and Gro1-4 gene markers were performed. Amplification products of the Gro1-4 gene marker were sequenced.Results. Only seven among the studied 34 potato genotypes (two of S. brachystotrichum (Bitt.) Rydb., four of S. lesteri Hawkes et Hjerting, and one of S. kurtzianum Bitt. et Wittm.) were susceptible to PGN, while the rest demonstrated high or medium resistance. Molecular screening for the presence of H1 and Gro1-4 gene markers allowed us to identify Gro1-4 in 13 South American genotypes of S. alandiae Cárd., S. × doddsii Corr., S. kurtzianum, S. leptophyes Bitt., and S. yungasense Hawkes. The remaining 14 genotypes may supposedly contain resistance genes non-identical to H1 or Gro1-4. Hybrids of S. tuberosum L. with medium-resistant wild accessions of S. kurtzianum, S. leptophyes, S. sparsipilum (Bitt.) Juz. et Buk., S. alandiae, and S. × doddsii inherited PGN resistance determined either by the Gro1-4 gene or genes non-identical to H1 or Gro1-4. Sequencing a fragment of the Gro1-4 gene showed that changes in the structure of this fragment in orthologous genes did not affect the feature of resistance to PGN pathotype Ro1.Conclusion. For the first time, sources of resistance to PGN were found among the North American species S. brachystotrichum (k-23201) and S. lesteri (k-24475). Among the wild South American Solanum spp., sources of resistance determined by genes different from H1 or Gro1-4 were identified. Resistant interspecific hybrids can serve as donors of the Gro1-4 resistance gene or new resistance genes.
Highlights
Predominant use of the H1 and Gro1-4 genes of resistance to golden nematode (PGN) in potato breeding requires widening the gene pool of resistance to this pathogen
Thirty-four genotypes of wild potatoes from North and South Americas, 14 interspecific hybrids, and 10 Russian potato cultivars were studied for PGN resistance
Molecular screening for the presence of H1 and Gro1-4 gene markers allowed us to identify Gro1-4 in 13 South American genotypes of S. alandiae Cárd., S. × doddsii Corr., S. kurtzianum, S. leptophyes Bitt., and S. yungasense Hawkes
Summary
Predominant use of the H1 and Gro genes of resistance to golden nematode (PGN) in potato breeding requires widening the gene pool of resistance to this pathogen. Hybrids of S. tuberosum L. with medium-resistant wild accessions of S. kurtzianum, S. leptophyes, S. sparsipilum (Bitt.) Juz. et Buk., S. alandiae, and S. Among the wild South American Solanum spp., sources of resistance determined by genes different from H1 or Gro were identified. Устойчивость к разным патотипам ЗКН обнаружена у образцов южноамериканских диких видов картофеля S. acaule Bitt., S. alandiae Cárd., S. berthaultii Hawkes, S. brevicaule Bitt., S. Новые источники устойчивости к ЗКН были выявлены также среди образцов североамериканских диких видов картофеля, таких как S. trifidum Corr., S. Вследствие этого выявление источников известных и новых генов устойчивости к ЗКН среди образцов диких видов и межвидовых гибридов картофеля из коллекции ВИР является актуальной задачей исследований.
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