Wild mushrooms showed analgesic and cytotoxic properties along with phytoconstituent's binding affinity to COX-1, COX-2 and cytochrome P450 2C9

S.M Moazzem Hossen,Mohammad Shahadat Hossain,Sumaiya Akbar,Umme Tahmida,S M Moazzem Hossen,Jannatul Mawa,Nazim Uddin Emon

doi:10.1016/j.heliyon.2021.e07997

S.M Moazzem Hossen, Mohammad Shahadat Hossain + Show 5 more

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https://doi.org/10.1016/j.heliyon.2021.e07997

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Abstract

This study was designed to evaluate the cytotoxic and analgesic potential of methanol extracts of five wild mushrooms available in the University of Chittagong, Bangladesh. The acetic acid-induced writhing method was used for the analgesic activity, while cytotoxicity was tested using brine shrimp lethality bioassay. In silico molecular docking and ADME/T study have been employed by using Schrodinger v11.1, BIOVIA Discovery Studio 2020 and online tool (AdmeSAR) respectively. The methanol extracts of Daldinia concentrica, Trametes lactinea, Fomitopsis cajanderi and Daedaleopsis confragosa exhibited a significant (p < 0.001) decrease in the number of writhing when compared to the control group. Except for Lentinus squarrosulus at 200 mg/kg body weight, the remaining mushroom extracts showed equal to or above 50 % inhibition of writhing. Daldinia concentrica showed the lowest LC50 values with 0.63 μg/mL, while Daedaleopsis confragosa showed the highest LC50 values of 2.33 μg/mL, indicating decisive cytotoxic action all mushrooms extracts. Considering the secondary metabolites, daldinan A and fomlactone A were found the most promising myco-compounds in analgesic and cytotoxic molecular docking studies. Besides, all the selected metabolites meet the rule of Lipinski's drug-likeliness. These results concluded that each mushroom extracts except Lentinus squarrosulus possess a potential analgesic. All the mushroom extracts embrace a promising cytotoxic activity that may guide the progress of a new drug.

Highlights

From ancient civilization to modern times, mushrooms have been used as food for their enormous nutritional values (Bhunia et al, 2010; Rathore et al, 2017)
Five wild mushrooms, including Lentinus squarrosulus, Daldinia concentrica, Trametes lactinea, Fomitopsis cajanderi and Daedaleopsis confragosa were collected from different areas of the Chittagong University campus, Bangladesh
Extracts with the LC50 value greater than 1000 μg/mL are recommended as non-toxic (Deciga-Campos et al, 2007)

Summary

Introduction

From ancient civilization to modern times, mushrooms have been used as food for their enormous nutritional values (Bhunia et al, 2010; Rathore et al, 2017). Mushrooms possess notable medicinal effects as the fungi (Gargano et al, 2017). Nowadays, they are widely utilized in the nutraceuticals, pharmaceuticals, and cosmeceuticals sector Rathore et al (2017). Previous research of Trametes lactinea (showed that this mushroom contains a biologically active constituent, namely Trametenolic acid B, which effectively suppressed the gastric cancer cells through H þ -K þ ATPase inhibitory activities (He et al, 2018; Zhang et al, 2013). On the dead, dying logs, the fruit bodies are individually or dispersed and identified This fungus is used to cure pneumonia and other bacterial infections as conventional therapies in Yorubaland, Western Nigeria (Goswami et al, 2020)

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