Abstract
In yeast, the chromatin remodeler Isw1 shifts nucleosomes from mid-coding, to more 5’ regions of genes and may regulate transcriptional elongation.
Highlights
The positions of nucleosomes along eukaryotic DNA are defined by the local DNA sequence and are further tuned by the activity of chromatin remodelers
We used Illumina high-throughput sequencing to map genome-wide nucleosome positioning in wild-type yeasts and in mutants deleted of ISW1 (Figure 1a)
An inter-species analysis and the evolutionary implications will be presented elsewhere while here we focus on the influence of deleting ISW1, which is largely conserved between the two species and observed in the hybrid
Summary
The positions of nucleosomes along eukaryotic DNA are defined by the local DNA sequence and are further tuned by the activity of chromatin remodelers. Nucleosome positioning is determined, to a large extent, by the local DNA sequence and its affinity to nucleosomes [5,6,7], but is dynamically altered by the activity of a large number of chromatin-associated proteins [8,9]. Transcription factors and other DNA-binding proteins can influence nucleosome positioning by competing with nucleosomes for binding to DNA [5,10]. Chromatin regulators directly modify the positions, or the states, of nucleosomes. Chromatin remodelers directly alter the histone-DNA contacts and are expected
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