Wide-field label-free imaging of thick tissues using optical diffraction tomography (Conference Presentation)

Abstract

Optical diffraction tomography (ODT) is a label-free three-dimensional (3D) microscopy technique allowing to retrieve the refractive-index distributions of optically translucent samples. Until recently, the histopathological applications of ODT have been impeded by the difficulty of imaging thick and wide tissues. Here, we demonstrate rapid, wide-field 3D ODT for imaging biological tissues. We designed a high-speed stitching ODT platform exploiting a digital micromirror device and a motorized stage. Developing a fast algorithmic framework that relieves the stitching artifacts and contrast loss due to multiple scattering, we demonstrate high-contrast 3D imaging of 100-m-thick, square-millimeter-wide pathological tissues at sub-micrometer resolution.