Abstract
Most reports on oblique plane microscopy (OPM) have a limited microscopic field of view (FOV), typically within 1x1 mm2. We implemented an optical design of mesoscopic scanning oblique plane microscopy (OPM) to allow using low numerical aperture (NA) objective lens. The angle of the intermediate image before the remote focusing system was increased by a demagnification under Scheimpflug condition such that the light collecting efficiency in the remote focusing system was significantly improved. We demonstrate a mesoscopic FOV up to ~6× 5×0.6 mm3 volumetric imaging, the largest reported FOV by OPM so far.
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