Abstract
We demonstrate a highly sensitive lensless fluorescence imaging device with a wide field-of-view by using a hybrid bandpass filter composed of interference filters, an absorption filter, and a fiber optic plate. The hybrid filter shows high excitation light rejection characteristics even in a lensless setup. In this study, we fabricated a hybrid bandpass filter and improved fluorescence observation performance for a target with auto-fluorescence. The filter was combined with a large image sensor with an imaging area of 67 mm2. As a demonstration, a brain slice from a green fluorescent protein transgenic mouse was observed and fluorescent cell bodies were detected with the lensless imaging device.
Highlights
Lensless microscopy makes it possible to build wide field-ofview devices with small dimensions due to its inherently simple optics.1–3 it is expected to be applied to various areas, for example, time-lapse imaging of culturing cells in the small space of a CO2 incubator4,5 or point-of-care devices with high portability
We demonstrated the improvement of green fluorescent protein (GFP) imaging performance by the effect of the bandpass characteristics of the new hybrid filter
We demonstrated a wide field-of-view lensless fluorescence imaging device with bandpass characteristics
Summary
Lensless microscopy makes it possible to build wide field-ofview devices with small dimensions due to its inherently simple optics. it is expected to be applied to various areas, for example, time-lapse imaging of culturing cells in the small space of a CO2 incubator or point-of-care devices with high portability. It is expected to be applied to various areas, for example, time-lapse imaging of culturing cells in the small space of a CO2 incubator or point-of-care devices with high portability. In lensless fluorescence imaging devices, it is typical to use an interference filter or absorption filter, as well as conventional lens-based fluorescence microscopes. Both of them have problems in a lensless setup. We proposed a novel “hybrid” emission filter that uses the advantages of the interference and absorption filters complementarily.. We proposed a novel “hybrid” emission filter that uses the advantages of the interference and absorption filters complementarily.16 This filter shows excitation light transmittance of approximately 10−8 with a lensless setup. Highly sensitive fluorescence detection performance similar to that for a lens-based fluorescent microscope has been achieved
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