Abstract
Incoherent correlation microscopy is recently discovered technique for digital imaging of three-dimensional objects in a quasi-monochromatic spatially incoherent light. Its operation is based on wavefront division carried out by a spatial light modulator and capturing correlation recordings of the observed scene. To achieve image reconstruction, at least a partial overlapping of the signal and reference waves created by the spatial light modulator is necessary. In the known experimental configurations, the overlapping of interfering beams is strongly reduced in off-axis areas of the object and the image can be reconstructed only in a very small portion of the field of view provided by the used microscope objective lens. Here, we propose and successfully demonstrate modified experimental system working with two-component relay optics inserted between the microscope objective and the spatial light modulator and providing full overlapping of correlated beams in all areas of the field of view of the objective lens. The benefits and applicability of the proposed system design are clearly demonstrated on the imaging of the USAF resolution targets.
Highlights
Digital holographic microscopy is a powerful imaging technique suitable for both metrology and bio-photonic applications including surface analysis or marker-free dynamic live cell imaging [1, 2]
The spatial light modulator (SLM) can be used in both illuminating and imaging paths of the optical microscope, where it ensures a structured specimen illumination, or amplitude and phase modulation of the spatial spectrum, respectively [4]. Using these phase modulation techniques, the spatial light interference microscopy (SLIM) [5, 6] and the spiral phase contrast imaging [7] were presented as powerful techniques capable of measuring nanoscale structures and dynamics in live cells or enhancing standard phase contrast methods
We showed that a significant extension of the field of view (FOV) is possible in the modified system using a two-component relay optics to achieve a perfect overlapping of the correlated beams
Summary
Digital holographic microscopy is a powerful imaging technique suitable for both metrology and bio-photonic applications including surface analysis or marker-free dynamic live cell imaging [1, 2]. It is based on the principles of optical holography, which are advantageously combined with the recording of the holograms by a digital image sensor. The SLM can be used in both illuminating and imaging paths of the optical microscope, where it ensures a structured specimen illumination, or amplitude and phase modulation of the spatial spectrum, respectively [4] Using these phase modulation techniques, the spatial light interference microscopy (SLIM) [5, 6] and the spiral phase contrast imaging [7] were presented as powerful techniques capable of measuring nanoscale structures and dynamics in live cells or enhancing standard phase contrast methods. In the common-path phase-shifting lensless holographic microscopy, the SLM was used for a wave multiplexing ensuring a holographic recording and digital reconstruction of the specimen [10]
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