Why nonlinear Raman microspectroscopy is more than just a gimmick?

Abstract

Coherent Raman microspectroscopy utilizing either stimulated Raman scattering or coherent Raman scattering is gaining popularity in the field of biological imaging. It is generally assumed that coherent Raman spectroscopy provides a stronger signal than conventional spectroscopy based on spontaneous scattering, allowing much faster imaging. In this report we examine non-trivial advantages of coherent Raman microspectroscopy, which haven’t been given significant attention lately. In particular, we demonstrate the potential of improving sensitivity of Raman spectroscopy and ability to sense the local nanoenvironment.