Whole-Genome Comparative Analysis Reveals Association Between Salmonella Genomic Variation and Egg Production Systems.

Ihab Habib,Hamid Reza Sodagari,Shafi Sahibzada,Ian Robertson,Penghao Wang

doi:10.3389/fvets.2021.666767

Ihab Habib, Hamid Reza Sodagari + Show 3 more

Open Access

https://doi.org/10.3389/fvets.2021.666767

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Abstract

Non-typhoidal Salmonella, particularly Salmonella enterica serovar Typhimurium (S. Typhimurium), is the predominant endemic serovar in the Australian egg production industry and is one of the most frequently reported serovars in foodborne infections in Australia. This study was conducted to investigate the genomic characteristics of Salmonella isolated from retail table eggs in Western Australia and to identify the impact of production systems on genomic characteristics of Salmonella such as virulence and antimicrobial resistance. A total of 40 non-typhoidal Salmonella isolates [S. Typhimurium isolates (n = 28) and Salmonella Infantis isolates (n = 12)] sourced from retail eggs produced by different production systems (barn-laid, cage, and free-range) in Western Australia were sequenced by whole-genome sequencing. The isolates were de novo assembled, annotated, and analyzed. The results indicated an association between Salmonella genomic variation and the system used to raise poultry for egg production (p-value < 0.05). All but one of the S. Infantis isolates were recovered from eggs collected from poultry raised under barn and cage production systems. A higher proportion (83.3%) of S. Typhimurium isolates were recovered from the eggs produced by free-range production system as compared with those produced under barn (76.9%) and cage production systems (53.3%). Our analysis indicated that Salmonella isolated from the eggs produced by barn and cage production systems had more virulence genes than the isolates of the free-range produced eggs. A low carriage of antimicrobial-resistant gene was detected in the isolates of this study. We have built a Salmonella genomics database and characteristics-linked gene pools to facilitate future study, characterization, and tracing of Salmonella outbreaks.

Highlights

Salmonella are gram-negative bacteria known for more than 100 years to cause foodborne illness in humans
A number of different Salmonella serovars have been isolated in humans and food animals in Australia; Salmonella enterica serovar Typhimurium
1,440 universal orthologs were searched against all chromosomes, with around 94.4% identified by BUSCO on average [44]

Summary

Introduction

Salmonella are gram-negative bacteria known for more than 100 years to cause foodborne illness in humans. Among 2,600 identified Salmonella serovars [2], non-typhoidal serovars have been recognized as the source of 550 million foodborne illnesses annually in the world with 230,000 deaths every year in the world [3]; many of these Salmonella-associated illnesses are preventable with appropriate interventions. In Australia, an estimated 5.4 million cases of foodborne disease occur annually, costing an estimated $1.2 billion per year [4]. A number of different Salmonella serovars have been isolated in humans and food animals in Australia; Salmonella enterica serovar Typhimurium Typhimurium in humans resulted in 2,343 cases with 347 requiring hospitalizations due to consumption of raw eggs and raw egg-related products in various food preparation settings across Australian states/territories, in New South Wales, Queensland, and Victoria [7]. A significant preference of Australian consumers for eggs from cagefree production systems (free-range and barn-laid) has been observed, due to the perception that non-cage production systems produce safer and higher-quality eggs [8] and to public concerns for animal welfare [9]

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