Abstract
BackgroundConditions leading to reduced gastric volume are difficult to manage and are associated to poor quality-of-life. Stomach augmentation using a tissue-engineered stomach is a potential solution to restore adequate physiology and food reservoir. Aim of this study was to evaluate the decellularisation of whole rat stomach using a detergent-enzymatic protocol.MethodsStomachs harvested from rats were decellularised through luminal and vascular cannulation using 24-h detergent-enzymatic treatment and completely characterized by appropriate staining, DNA and Extracellular matrix -component quantifications.ResultsThe detergent-enzymatic protocol allows a complete decellularisation of the gastric tissue, with a complete removal of the DNA with two cycles as confirmed by both quantifications and histological analysis. Extracellular matrix components, collagen, fibronectin, laminin and elastin, were optimally preserved by the treatment, while glycosaminoglycans were reduced.ConclusionGastric tissue can be efficiently decellularised. Scaffolds retained original structure and important components that could enhance integration with other tissues for in vivo transplant. The use of naturally derived material could be potentially considered for the treatment of both congenital and acquired conditions.
Highlights
Congenital and acquired conditions can lead to a reduced volume of the stomach
Aim of this paper is to develop a decellularisation protocol to efficiently create a scaffold suitable for stomach augmentation
Quantification of collagen revealed an increase in its relative content (Fig. 4a) while elastin was preserved (Fig. 4b) and a reduction was observed in glycosaminoglycans (Fig. 4c)
Summary
Congenital and acquired conditions can lead to a reduced volume of the stomach. Gastric tissue is widely used in the surgical treatment of congenital and acquired childhood diseases involving the oesophagus. Conditions leading to reduced gastric volume are difficult to manage and are associated to poor quality-of-life. Aim of this study was to evaluate the decellularisation of whole rat stomach using a detergent-enzymatic protocol. Methods Stomachs harvested from rats were decellularised through luminal and vascular cannulation using 24-h detergentenzymatic treatment and completely characterized by appropriate staining, DNA and Extracellular matrix -component quantifications. Results The detergent-enzymatic protocol allows a complete decellularisation of the gastric tissue, with a complete removal of the DNA with two cycles as confirmed by both quantifications and histological analysis. The use of naturally derived material could be potentially considered for the treatment of both congenital and acquired conditions
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