Abstract
Human adenoviruses (HAdVs) are prevalent in patients with respiratory infections, in which recombination has important implications for viral detection and pathogenicity. However, less HAdVs recombination was reported in Qinghai plateau. In this study, we obtained an HAdV-C strain (QH-1665/2018) isolated from an infant aged one month with influenza-like illness in Qinghai Province in 2018. The whole genome sequence was generated by next-generation sequencing, and compared with that of other HAdV-C strains available in public. The strain QH-1665/2018 genome is comprised of 36,014 nucleotides and encoded 36 putative proteins. Phylogenetic analysis of complete HAdV genomes and 3 major antigen genes (penton, hexon and fiber) showed that strain QH-1665/2018 was clustered into HAdV-1 [P1H1F1]. Recombination analysis based on the RDP4 package and SimPlot software showed that QH-1665/2018 was a recombinant involving HAdV-1, HAdV-2 and HAdV-5, which was then re-confirmed by phylogenetic analysis. Our results suggest that HAdV-C recombination is highly complex, should be focused on, and the epidemiological and virological surveillance should be strengthened in Qinghai Province.
Highlights
Adenovirus is a non-enveloped, double-stranded DNA virus belonging to the Adenoviridae family, that can cause infections related to the respiratory tract, gastrointestinal tract and eyes in humans [1]
Whole genome phylogenetic analysis of complete human adenoviruses (HAdVs) genomes illustrated that strain QH-1665/2018 was clustered into HAdV-1, and phylogenetic analysis of penton, hexon and fiber genes showed that the 3 major antigen genes were classified into P1, H1, and F1 (Fig. 1)
We found that the 52 K gene showed the highest sequence similarity with HAdV-2, the E4 gene showed the highest sequence similarity with HAdV-5, the pTP and protease gene showed the highest sequence similarity with HAdV-6, the E1B region showed the highest sequence similarity with HAdV-57, and the pVII gene showed the highest similarity with both HAdV-6 and HAdV-57
Summary
Adenovirus is a non-enveloped, double-stranded DNA virus belonging to the Adenoviridae family, that can cause infections related to the respiratory tract, gastrointestinal tract and eyes in humans [1]. Complete genomic data have enhanced the understanding of HAdV epidemiology and is an important way to recognize the recombination in HAdV pathogens. Until now, little data on HAdV recombination have been reported in Qinghai Province. We describe the characterization of an HAdV-1 recombinant (QH-1665/2018) isolated from an infant aged one month with influenza-like illness in Qinghai Province in 2018. This information would enhance the understanding of recombination of HAdV-C, and might assist with effective prevention and control of respiratory adenovirus infection in Qinghai Province
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