Abstract
Lactobacillus is a diverse genus that includes species of industrial and biomedical interest. Lactiplantibacillus pentosus, formerly known as Lactobacillus pentosus, is a recently reclassified species, that contains strains isolated from diverse environmental niches, ranging from fermented products to mammalian gut microbiota. Importantly, several L. pentosus strains present health-promoting properties, such as immunomodulatory and antiproliferative activities, and are regarded as potential probiotic strains. In this study, we present the draft genome sequence of the potential probiotic strain L. pentosus L33, originally isolated from fermented sausages. Comprehensive bioinformatic analysis and whole-genome annotation were performed to highlight the genetic loci involved in host-microbe interactions and the probiotic phenotype. Consequently, we found that this strain codes for bile salt hydrolases, adhesins and moonlighting proteins, and for Class IIb bacteriocin peptides lacking the GxxxG and GxxxG-like motifs, crucial for their inhibitory activity. Its adhesion ability was also validated in vitro, on human cancer cells. Furthermore, L. pentosus L33 contains an exopolysaccharide (EPS) biosynthesis cluster, and it does not carry transferable antibiotic resistance genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and CAZymes analyses showed that L. pentosus L33 possesses biosynthetic pathways for seven amino acids, while it can degrade a wide array of carbohydrates. In parallel, Clusters of Orthologous Groups (COGs) and KEGG profiles of L. pentosus L33 are similar to those of 26 L. pentosus strains, as well as of two well documented L. plantarum probiotic strains. Conclusively, L. pentosus L33 exhibits good probiotic potential, although further studies are needed to elucidate the extent of its biological properties.
Highlights
Lactobacillus is a diverse genus that includes Gram-positive, facultatively anaerobic, non-spore-forming, hetero, or homofermentative bacteria that inhabit a broad range of nutrientrich environmental niches (Duar et al, 2017)
We present the draft genome sequence of Lactiplantibacillus pentosus L33, a strain isolated from traditional meat products (Pavli et al, 2016)
The genetic traits that can be affected by this event are clusters for amino acid synthesis, and genes involved in metabolism regulation (Zheng et al, 2015)
Summary
Lactobacillus is a diverse genus that includes Gram-positive, facultatively anaerobic, non-spore-forming, hetero-, or homofermentative bacteria that inhabit a broad range of nutrientrich environmental niches (Duar et al, 2017). Lactobacillus strains can be found as autochthonous or allochthonous, mainly in the mammalian gastrointestinal tract, fresh fruit and vegetable microbiota, as well as in fermented foodstuffs (Inglin et al, 2018). In this context, several strains exhibit great biotechnological interest, due to their fermentation capacity and are being incorporated as starter cultures in a broad range of dairy and non-dairy products (Kok and Hutkins, 2018). Probiotics are commercially available in supplements or in functional products, comprising a rapidly growing global market, currently worth more than $50 billion, as market reports indicated.
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