Abstract
Sphingobium sp. strain RSMS was described earlier as an efficient degrader of tributyl phosphate, an organic pollutant. This report describes the generation and annotation of the genome sequence of Sphingobium sp. strain RSMS, which will facilitate future studies to identify genetic elements responsible for the degradation of tributyl phosphate.
Highlights
Widespread use of large volumes (;3,000 to 5,000 tons/year) of tributyl phosphate (TBP) in the nuclear industry for extraction of uranium and plutonium [1] and in other industries [2] has resulted in large volumes of TBP-containing waste, which is an environmental hazard
A total of 11,610,225 paired-end 100-bp reads were generated from an Illumina HiSeq 2500 system, and low-quality reads were filtered using Trimmomatic v0.39 [9] with custom filters (Q score, .15 in a sliding window of 4 nucleotides [nt]; minimum length, .36 nt)
A total of 80,396 long reads were obtained with a MinION flow cell (MIN106D)
Summary
Widespread use of large volumes (;3,000 to 5,000 tons/year) of tributyl phosphate (TBP) in the nuclear industry for extraction of uranium and plutonium [1] and in other industries [2] has resulted in large volumes of TBP-containing waste, which is an environmental hazard. The whole-genome library was constructed using the Illumina TruSeq Nano DNA LT sample preparation kit set B according to the manufacturer’s instructions. A total of 11,610,225 paired-end 100-bp reads were generated from an Illumina HiSeq 2500 system, and low-quality reads were filtered using Trimmomatic v0.39 [9] with custom filters (Q score, .15 in a sliding window of 4 nucleotides [nt]; minimum length, .36 nt).
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