Whole-Genome Sequencing of Mycobacterium tilburgii Strain MEPHI.

Jamal Saad,Patrick J Stapleton,Simon Grandjean Lapierre,Margaret M Hannan,Michel Drancourt,Catherine Putonti

doi:10.1128/mra.00933-19

Abstract

Mycobacterium tilburgii is a fastidious mycobacterium which has previously been reported to cause severe disseminated infections. Genome sequencing of the M. tilburgii MEPHI clinical isolate yielded 3.14 Mb, with 66.3% GC content, and confirmed phylogenetic placement within the Mycobacterium simiae complex.

Highlights

13 cases of Mycobacterium tilburgii infection have been reported, highlighting host immunosuppression as a risk factor for M. tilburgii infection [1,2,3]
M. tilburgii was never previously isolated in culture; its detection relied on the direct sequencing of internal transcribed spacer 1 (ITS1), hsp65, rpoB, and 16S rRNA genes from clinical material [2]
Phylogenetic trees based on the 3,504-bp rpoB and the 1,523-bp 16S RNA genes confirmed phylogenetic placement into the Mycobacterium simiae complex (Fig. 1B and C)

Summary

Introduction

13 cases of Mycobacterium tilburgii infection have been reported, highlighting host immunosuppression as a risk factor for M. tilburgii infection [1,2,3]. Mycobacterium tilburgii is a fastidious mycobacterium which has previously been reported to cause severe disseminated infections. Genome sequencing of the M. tilburgii MEPHI clinical isolate yielded 3.14 Mb, with 66.3% GC content, and confirmed phylogenetic placement within the Mycobacterium simiae complex. M. tilburgii was never previously isolated in culture; its detection relied on the direct sequencing of internal transcribed spacer 1 (ITS1), hsp65, rpoB, and 16S rRNA genes from clinical material [2].

Results

Conclusion