Abstract
BACKGROUNDLeptospirosis is the most widespread zoonotic disease. It is caused by infection with pathogenic Leptospira species, of which over 300 serovars have been described. The accurate identification of the causative Leptospira spp. is required to ascertain the pathogenic status of the local isolates.OBJECTIVESThis study aimed to obtain the complete genome sequence of a virulent Leptospira interrogans strain isolated from southern Brazil and to describe its genetic features.METHODSThe whole genome was sequenced by next-generation sequencing (Ion Torrent). The genome was assembled, scaffolded, annotated, and manually reviewed. Mutations were identified based on a variant calling analysis using the genome of L. interrogans strain Fiocruz L1-130 as a reference.FINDINGSThe entire genome had an average GC content of 35%. The variant calling analysis identified 119 single nucleotide polymorphisms (SNPs), from which 30 led to a missense mutation. The structural analyses identified potential evidence of genomic inversions, translocations, and deletions in both the chromosomes.MAIN CONCLUSIONSThe genome properties provide comprehensive information about the local isolates of Leptospira spp., and thereby, could facilitate the identification of new targets for the development of diagnostic kits and vaccines.
Highlights
Leptospirosis is the most widespread zoonotic disease
Genome properties - The whole-genome shotgun sequencing of Piscina resulted in 689,451 reads with mean Phred score (Q) > 20, which represented coverage of ~30× when an L. interrogans genome of ~4.60 Mb, such as that of the Fiocruz L1-130 strain, was considered
The lack of coverage in some regions may be a consequence of the drawbacks associated with de novo genome assembly from short reads NGS data, the presence of repetitive DNA elements and transposases, and the existence of regions in the genome with large variations in the CG content that may be over- or under-fragmented during the library construction
Summary
This study aimed to obtain the complete genome sequence of a virulent Leptospira interrogans strain isolated from southern Brazil and to describe its genetic features
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