Whole-Genome Sequencing of a Campylobacter jejuni Strain Isolated from Retail Chicken Meat Reveals the Presence of a Megaplasmid with Mu-Like Prophage and Multidrug Resistance Genes.

Daya Marasini,Mohamed K Fakhr

doi:10.1128/genomea.00460-16

Abstract

Genome sequencing of Campylobacter jejuni strain T1-21 isolated from retail chicken meat revealed the presence of a chromosome of 1,565,978 bp and a megaplasmid of 82,732 bp that contains Mu-like prophage and multidrug resistance genes. This is the first reported sequence of a Campylobacter megaplasmid >55 kb.

Highlights

Genomic DNA was isolated from a microaerophilic 72-h culture grown in Muller-Hinton broth with 5% laked horse blood using the DNeasy blood and tissue kit (Qiagen, Valencia, CA)
In the last few years, our laboratory was successful in isolating a large number of C. jejuni and C. coli strains from retail chicken livers and gizzards (2), beef livers (3) and poultry meat (4)
We report here the whole-genome sequence of C. jejuni T1-21, which was isolated from retail chicken meat and shown by agarose gel electrophoresis to contain a large plasmid of approximately 80 kb in size

Summary

Introduction

Genomic DNA was isolated from a microaerophilic 72-h culture grown in Muller-Hinton broth with 5% laked horse blood using the DNeasy blood and tissue kit (Qiagen, Valencia, CA). Campylobacter jejuni and Campylobacter coli are the two major species associated with human infections (1). In the last few years, our laboratory was successful in isolating a large number of C. jejuni and C. coli strains from retail chicken livers and gizzards (2), beef livers (3) and poultry meat (4).

Results

Conclusion