Abstract
To analyze the whole genome of Bacillus aryabhattai strain SK1-7 and explore its potassium solubilization characteristics and mechanism, thus providing a theoretical basis for analyzing the utilization and improvement of insoluble potassium resources in soil. Genome information for Bacillus aryabhattai SK1-7 was obtained by using Illumina NovaSeq second-generation sequencing and GridION Nanopore ONT third-generation sequencing technology. The contents of organic acids and polysaccharides in fermentation broth of Bacillus aryabhattai SK1-7 were determined by high-performance liquid chromatography and the anthrone sulfuric acid method, and the expression levels of the potassium solubilization-related genes ackA, epsB, gltA, mdh and ppc were compared by real-time fluorescence quantitative PCR under different potassium source culture conditions. The whole genome of the strain consisted of a complete chromosome sequence and four plasmid sequences. The sequence sizes of the chromosomes and plasmids P1, P2, P3 and P4 were 5,188,391 bp, 136,204 bp, 124,862 bp, 67,200 bp and 12,374 bp, respectively. The GC contents were 38.2, 34.4, 33.6, 32.8, and 33.7%. Strain SK1-7 mainly secreted malic, formic, acetic and citric acids under culture with an insoluble potassium source. The polysaccharide content produced with an insoluble potassium source was higher than that with a soluble potassium source. The expression levels of five potassium solubilization-related genes with the insoluble potassium source were higher than those with the soluble potassium source.
Highlights
Potassium-solubilizing bacteria (KSB), known as silicate bacteria, are bacteria isolated from soil that can decompose aluminosilicate and apatite minerals
The whole genome of the strain consisted of a circular chromosome and four circular plasmid (Figure 1)
Strain SK1-7 was found to four plasmids, P1, P2, P3 and P4, with sequence sizes of 136,204 bp, 124,862 bp, 67,200 bp and 12,374 bp, and the GC contents were 34.4, 33.6, 32.8, and 33.7%, a total of 348 ORFs were predicted in all plasmid of which 313 were putative protein-coding DNA sequences (CDS), respectively (Table 3)
Summary
Potassium (K) is an essential element for plant nutrition and plays an important role in the growth and metabolism of plants. Potassium-solubilizing bacteria (KSB), known as silicate bacteria, are bacteria isolated from soil that can decompose aluminosilicate and apatite minerals. These organisms can transform insoluble potassium, phosphorus, silicon and other substances in soil into soluble substances and have the advantages of offering environmental protection with low cost and high efficiency (Ahmad et al, 2016; Bagyalakshmi et al, 2017; Ji et al, 2017; Khanghahi et al, 2018; Bahadur et al, 2019). In addition to transforming insoluble potassium in soil into soluble potassium that can be absorbed by plants, KSB can improve the physical and chemical properties of soil and secrete hormones that promote plant growth to increase plant biomass (Zhang and Kong, 2014)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
