Whole-Genome Sequences of Salmonella enterica subsp. enterica Serovar Typhimurium Strains TT6675 and TT9097 Employed in the Isolation and Characterization of a Giant Phage Mutant Collection.

Han Ming Gan,Nurul Aisyah Samsudin,Austin J Bartl,Michael A Savka,Julie A Thomas,André O Hudson,Lily E Adams,Melissa K Barton,Wilhelm Wei Han Eng

doi:10.1128/genomea.00857-17

Abstract

ABSTRACTWe report here the genome sequences of Salmonella enterica subsp. enterica serovar Typhimurium strains TT6675 and TT9097, which we utilize for genetic analyses of giant bacterial viruses. Our analyses identified several genetic variations between the two strains, most significantly confirming strain TT6675 as a serine suppressor and TT9097 as a nonsuppressor.

Highlights

We report here the genome sequences of Salmonella enterica subsp. enterica serovar Typhimurium strains TT6675 and TT9097, which we utilize for genetic analyses of giant bacterial viruses
Strain TT6675 was determined to encode standard tRNAs, whereas strain TT9097 was determined to encode standard tRNAs. This variation was due to the identification of a single nucleotide mutation in the anticodon (CGA-CTA) of the serine tRNA in TT6675, which produces a suppressor tRNA
Nonsuppressor phenotypes observed for TT6675 and TT9097, respectively

Summary

Introduction

We report here the genome sequences of Salmonella enterica subsp. enterica serovar Typhimurium strains TT6675 and TT9097, which we utilize for genetic analyses of giant bacterial viruses. Cells were cultured in nutrient medium, and genomic DNA was isolated using the GenElute bacterial genomic kit (Sigma-Aldrich, St. Louis, MO, USA). Sequencing libraries were prepared using the Nextera XT library prep kit (Illumina, San Diego, CA, USA) and tagged with unique dual-index barcodes. Sequencing was performed on the MiSeq desktop sequencer (Illumina) located at the Monash University Malaysia Genomics Facility.

Results

Conclusion