Whole genome sequence of Porphyromonas pogonae PP01-1, ahuman strain harboring blaOXA-347 and tet(Q)with chromosomal location

Abstract

Objectives: The aim of this study was to characterise the first complete genome of Porphyromonas pogonae strain PP01-1 of human origin in China. Methods: Illumina NovaSeq 6000 (200X coverage) and Nanopore MinION platform (100X coverage) were used for genome sequencing. The de novo hybrid assembly of short Illumina reads and long MinION reads was performed using Unicycler (v.0.5.0). Genome annotation of PP01-1 was performed using the prokaryotic gene prediction tool Prokka1.14.6.The genome was further analysed using several bioinformatics tools, including ResFinder, VFDB, VirulenceFinder,TYGS, AntiSMASH, PathogenFinder, MobileElementfinder, CRISPRFinder, and IslandViewer. Results: The assembled circular genome of Porphyromonas pogonae strain PP01-1 was 2,916,423 bp in length with a GC content of 41.0%, and no plasmid sequence was detected. A total of 2399 coding sequences were predicted by Prokka. PP01-1 harbors antimicrobial resistance genes blaOXA-347 (beta-lactamase resistance), tet(Q) (tetracycline) and floR (chloramphenicol and florfenicol). Conclusion: Here we first report the complete genome of Porphyromonas pogonaestrain PP01-1 of human origin. In this strain, we firstly identified blaOXA-347 and tet(Q) in Porphyromonas pogonae, which will paves the way of further analysis that would aim to identify the potential mechanism of ARGs and virulence factors in Porphyromonas pogonae.