Abstract

We have identified the whole-genome sequence of Lactobacillus salivarius DJ-sa-01, a potential probiotic strain for poultry, isolated from a chicken small intestine. We used the PacBio and Illumina platforms to obtain the sequence of the entire single circular chromosome.

Highlights

  • Plants produce a large number of specialized metabolites that are crucial for their interactions with the ever-changing environment (Keurentjes, 2009; Saito and Matsuda, 2010)

  • To evaluate the extent of variation of PA types and levels in rice leaf and seed, we measured the levels of non-spermidine-based PAs with different amine moieties, such as agmatine, putrescine, and tryptamine, by liquid chromatography-mass spectrometry (LC-MS)based targeted metabolic profiling using samples from a diverse worldwide collection of 480 rice accessions

  • We found three synonymous single-nucleotide polymorphisms (SNPs), an insertion of four consecutive amino acids, together with a 7-bp deletion that resulted in a frame shift in Os11g42370 were termed tryptamine benzoyl transferase 1 (Os-TBT1) in MH63 compared with the same gene in ZS97 (Figure 6C; Supplemental Data Set 4)

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Summary

Introduction

Plants produce a large number of specialized (secondary) metabolites that are crucial for their interactions with the ever-changing environment (Keurentjes, 2009; Saito and Matsuda, 2010). Much of the enormous diversity of specialized metabolites in plants comes from modifications or decorations of core structures by different tailing reactions such as acylation (Bontpart et al, 2015), glycosylation (Bowles et al, 2005), methylation (Lam et al, 2007), and so on (Schwab, 2003). Phenolamides (PAs), referred to as hydroxycinnamic acid amides or phenylamides, are the hydroxycinnamoyl acylated. Condensation of the hydroxycinnamoyl and amine moieties is the key step for the biosynthesis of PAs and is catalyzed by a diversity of hydroxycinnamoyl transferases (Petersen, 2015). Eleven rice phenolamides in this study refer to different combinations of CoA-thioesters and amine moieties

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