Abstract
The spread of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae is a global concern. The management of infections caused by multidrug resistance (MDR) isolates poses substantial clinical challenges in both hospitals and communities. This study aimed to investigate the genetic characteristics and variations of MDR E. coli and K. pneumoniae isolates. Bacterial identification and antibiotic susceptibility testing against 19 antibiotics were performed by standard methods. Whole genome sequencing (WGS) was carried out on eight carbapenem-resistant isolates using an Illumina MiSeq platform. The assembled draft genomes were annotated, then sequences were blasted against antimicrobial resistance (AMR) genes database. WGS detected several resistance genes mediating the production of β-lactamases, including carbapenems and extended-spectrum β-lactamase genes as (blaOXA-1/-48, blaKPC-2/-29, blaCMY-4/-6, blaSHV-11/-12, blaTEM-1, blaCTX-M-15, blaOKP-B, blaACT and blaEC). Furthermore quinolone resistance including oqxA/oqxB, aac(6′)-Ib-cr5, gyrA_D87N, gyrA_S83F, gyrA_S83L, parC_S80I, parE_S458A, parE_I355T, parC_S80I, and qnrB1. In addition to aminoglycoside modifying enzymes genes (aph(6)-Id, aph(3″)-Ib, aac(3)-IIa, aac(6′)-Ib, aadA1, aadA2 and aadA5), trimethoprim-sulfamethoxazole (dfrA12/A14/A17 and sul1/sul2), tetracycline (tetA and tetB), fosfomycin (fosA and uhpT_E350Q) resistance genes, while other genes were detected conferring chloramphenicol (floR, catA2, and efflux pump cmIA5), macrolides resistance (mph(A) and erm(B), and quaternary ammonium efflux pump qacEdelta. Bleomycin and colistin resistance genes were detected as ble and pmrB_R256G, respectively. Comprehensive analysis of MDR strains provided by WGS detected variable antimicrobial resistance genes and their precise resistance mechanism. WGS is essential for control and prevention strategies to combat the growing threat of AMR and the implementation of multifaceted interventions are needed.
Highlights
Publisher’s Note: MDPI stays neutralThe escalating burden of antimicrobial resistance worldwide is substantial and is likely to grow [1]
The emergence and spread of multidrug resistance (MDR) K. pneumoniae and E. coli isolates complicate the treatment of severe infections and threaten to create strains of bacteria resistant to currently with regard to jurisdictional claims in published maps and institutional affiliations
Two isolates belonged to Canadian and Kuwaiti patients who were admitted to Mubarak hospital (MK) in Hawali Governorate and Farwaniya hospital (FA) in Farwaniya Governorate, respectively
Summary
Publisher’s Note: MDPI stays neutralThe escalating burden of antimicrobial resistance worldwide is substantial and is likely to grow [1]. The emergence of multidrug resistance (MDR) Enterobacterales such as Klebsiella pneumoniae and Escherichia coli is a well-known global health concern and possesses numerous resistance genes in its genome [3]. Carbapenems are the drug of choice and are the most potent group of β-lactam antibiotics used to treat serious infections caused by ESBL-producing Enterobacterales and AmpC β-lactamase producers. They are frequently used for empiric therapy of life-threatening infections, such as sepsis [4,5]. The emergence and spread of MDR K. pneumoniae and E. coli isolates complicate the treatment of severe infections and threaten to create strains of bacteria resistant to currently with regard to jurisdictional claims in published maps and institutional affiliations
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