Abstract
Cupriavidus sp. are model organisms for heavy metal(loid) resistance and aromatic compound’s degradation studies and these characteristics make them a perfect candidate for biotechnological purposes. Bacterial strain S14E4C (identified as Cupriavidus campinensis) was isolated from a playground by enrichment method in a 0.25 mM containing medium. The analysis revealed that this bacterium is able to tolerate high concentrations of heavy metal(loid)s: Cd up to 19.5 mM, Pb to 9 mM, Hg to 5.5 mM and As to 2 mM in heavy metal(loid) salt containing nutrient medium. The whole genome data and analysis of the type strain of C. campinensis CCUG:44526T have not been available so far, thus here we present the genome sequencing results of strain S14E4C of the same species. Analysis was carried out to identify possible mechanisms for the heavy metal resistance and to map the genetic data of C. campinensis. The annotation pipelines revealed that the total genome of strain S14E4C is 6,375,175 bp length with a GC content of 66.3% and contains 2 plasmids with 295,460 bp (GC content 59.9%) and 50,483 bp (GC content 63%). In total 4460 coding sequences were assigned to known functions and 1508 to hypothetical proteins. Analysis proved that strain S14E4C is having gene clusters such as czc, mer, cus, chr, ars to encode various heavy metal resistance mechanisms that play an important role to survive in extreme environments.
Highlights
Extensive use of metals and chemicals in the industrial processes have resulted in accumulation of large quantities of effluents containing toxic heavy metals in the environment, and these effluents pose environmental disposal problems due to their non-degradable and persistent characters [1]
The β-proteobacterium Cupriavidus campinensis was isolated first time from Campine, the geographical region of northeast Belgium [3]. This bacterium was found to be highly resistant to heavy metals [3] and following analysis confirmed the ability of degradation 2,4-dichlorophenoxyacetic acid by other strain [4] as well, as the extrachromosomal genetic determinants were transferable to related bacteria
The 16S rRNA gene sequence of strain S14E4C (NCBI GenBank accession MK660715) was obtained and BLAST search results based on EzTaxon and GenBank databases both indicated that strain S14E4C belongs to the genus Cupriavidus and it is 100% identical to Cupriavidus campinensis WS2 [3]
Summary
Extensive use of metals and chemicals in the industrial processes have resulted in accumulation of large quantities of effluents containing toxic heavy metals in the environment, and these effluents pose environmental disposal problems due to their non-degradable and persistent characters [1]. The β-proteobacterium Cupriavidus (formerly Wautersia, Ralstonia) campinensis was isolated first time from Campine, the geographical region of northeast Belgium [3]. This bacterium was found to be highly resistant to heavy metals [3] and following analysis confirmed the ability of degradation 2,4-dichlorophenoxyacetic acid by other strain [4] as well, as the extrachromosomal genetic determinants were transferable to related bacteria. Molecular Biology Reports (2020) 47:3973–3985 was sequenced and analysed in detail, as well as heavy metal calculated by coverage calculator master v0.0.1 Molecular Biology Reports (2020) 47:3973–3985 was sequenced and analysed in detail, as well as heavy metal calculated by coverage calculator master v0.0.1 (https://githuresistance genes and genomic potentials were characterised. b.com/GenomicaMicrob/coverage_calculator)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
